Review



akta start fplc system  (Cytiva Europe)


Bioz Verified Symbol Cytiva Europe is a verified supplier
Bioz Manufacturer Symbol Cytiva Europe manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    Cytiva Europe akta start fplc system
    Akta Start Fplc System, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 96/100, based on 683 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/akta+start+fplc+system/AKTA+start+chromatography+system/pm41500280-79-18-22
    Average 96 stars, based on 683 article reviews
    akta start fplc system - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    Suspension:

    Article Title: Low collision energy fragmentation in structure-specific glycoproteomics analysis
    Article Snippet: Glycoproteins Recombinant Human PD-L1/B7-H1 Fc Chimera Protein overexpressed in Mouse myeloma (NS0) cell line (R&D Systems, Minneapolis, MN); Recombinant Human PD-L1/B7-H1 His-tag Protein overexpressed in Human embryonic kidney cell (HEK 293) cell line (R&D Systems), Human Haptoglobin, mixed type isolated from plasma (Athens Research & Technology, Athens, GA), Human Hemopexin isolated from plasma (Athens Research & Technology). .. His-tagged ProBDNF was expressed in suspension HEK293F cells (Invitrogen, Carlsbad, CA) and purified using Ni-NTA resin (Thermo, Rockford, IL) chromatography on AKTA Start FPLC system (GE Healthcare, Uppsala, Sweden) to > 90% purity as determined by Coomassie Blue stained SDS-PAGE as described 24 Lyophilized proteins were dissolved in 100 mM PBS at a concentration of 0.5 μg/ml and stored at −80°C. ..

    Purification:

    Article Title: Low collision energy fragmentation in structure-specific glycoproteomics analysis
    Article Snippet: Glycoproteins Recombinant Human PD-L1/B7-H1 Fc Chimera Protein overexpressed in Mouse myeloma (NS0) cell line (R&D Systems, Minneapolis, MN); Recombinant Human PD-L1/B7-H1 His-tag Protein overexpressed in Human embryonic kidney cell (HEK 293) cell line (R&D Systems), Human Haptoglobin, mixed type isolated from plasma (Athens Research & Technology, Athens, GA), Human Hemopexin isolated from plasma (Athens Research & Technology). .. His-tagged ProBDNF was expressed in suspension HEK293F cells (Invitrogen, Carlsbad, CA) and purified using Ni-NTA resin (Thermo, Rockford, IL) chromatography on AKTA Start FPLC system (GE Healthcare, Uppsala, Sweden) to > 90% purity as determined by Coomassie Blue stained SDS-PAGE as described 24 Lyophilized proteins were dissolved in 100 mM PBS at a concentration of 0.5 μg/ml and stored at −80°C. ..

    Article Title: Structure, dynamics, and molecular inhibition of the Staphylococcus aureus m 1 A22-tRNA methyltransferase TrmK.
    Article Snippet: .. Cells were allowed to grow for an additional 20 h, harvested by centrifugation at 6774g for 15 min, and stored at −20 C. Purification of SaTrmK All purification procedures were carried out at 4 C, and chromatographic steps employed an AKTA Start FPLC system (GE Lifesciences). .. Cells were allowed to thaw on ice for 20 min before being resuspended in buffer A (50 mM Hepes, 10 mM imidazole, 300 mM NaCl, pH 8.0) containing 0.2 mg mL−1 lysozyme, 0.05 mg mL−1 DNAse I, and half a tablet of EDTA-free cOmplete Protease Inhibitor Cocktail, disrupted in a high-pressure cell disruptor (Constant Systems), and centrifuged at 48,000g for 30 min to remove cell debris.

    Article Title: A 6-O-endosulfatase activity assay based on synthetic heparan sulfate oligomers.
    Article Snippet: A 6-O-endosulfatase activity assay based on synthetic heparan sulfate oligomers Julius Benicky1,2, Miloslav Sanda3,4, Aswini Panigrahi1,2, Jian Liu5, Zhangjie Wang5, Vijayakanth Pagadala6, Guowei Su6, Radoslav Goldman1,2,3,* 1Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC 20057, United States, 2Clinical and Translational Glycoscience Research Center, Georgetown University, Washington, DC 20057, United States, 3Department of Biochemistry and Molecular & Cell Biology, Georgetown University, Washington, DC 20057, United States, 4Max-Planck-Institut fuer Herzund Lungenforschung, Ludwigstrasse 43, Bad Nauheim 61231, Germany, 5Division of Chemical Biology and Medicinal Chemistry, Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, NC 27599, United States, 6Glycan Therapeutics, LLC, 617 Hutton Street, Raleigh, NC 27606, United States *Corresponding author: Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, 3970 Reservoir Rd NW, NRB-E207, Washington, DC 20057, United States.. Email: rg26@georgetown.edu

    Article Title: Functional and ligand binding studies of NAD(P)H hydrate dehydratase enzyme from vancomycin-resistant Staphylococcus aureus by NMR spectroscopic approach, including saturation transfer difference (STD-NMR) spectroscopy.
    Article Snippet: NADH and NADPH are labile coenzymes that undergo hydration by enzymatic reaction or by heat at 5,6 double bond, and convert into non-functional hydrates, NADHX and NADPHX, respectively.. The NAD(P)H hydrate dehydratase enzyme catalyzes the dehydration of S-NADHX/S-NADPHX at the expense of ATP, and thus contributes in the nicotinamide nucleotide repair process.. This enzyme is also known as “metabolite-proofreading enzyme”.

    Article Title: HLA class I signal peptide polymorphism determines the level of CD94/NKG2–HLA-E-mediated regulation of effector cell responses
    Article Snippet: In brief, constructs encoding the chimeric proteins with a His6 tag, utilizing the pHLSec-Avitag3 construct backbone, were transfected into Expi293F suspension cells according to the manufacturer’s protocols (Expi293 Expression System, Thermo Fisher Scientific). .. After 5 days of expression, the supernatant was harvested, clarified by centrifugation and filtration, and the tagged proteins purified by immobilized metal affinity chromatography utilizing a Ni-NTA column attached to an Akta start FPLC system (Cytiva Life Sciences) followed by size-exclusion chromatography using a Superdex S200 increase column, equilibrated with 150 mM NaCl 20 mM Tris pH 8. ..

    Article Title: HLA class I signal peptide polymorphism determines the level of CD94/NKG2-HLA-E-mediated regulation of effector cell responses.
    Article Snippet: Human leukocyte antigen (HLA)-E binds epitopes derived from HLA-A, HLA-B, HLA-C and HLA-G signal peptides (SPs) and serves as a ligand for CD94/NKG2A and CD94/NKG2C receptors expressed on natural killer and T cell subsets.. We show that among 16 common classical HLA class I SP variants, only 6 can be efficiently processed to generate epitopes that enable CD94/NKG2 engagement, which we term ‘functional SPs’.. The single functional HLA-B SP, known as HLA-B/−21M, induced high HLA-E expression, but conferred the lowest receptor recognition.

    Chromatography:

    Article Title: Low collision energy fragmentation in structure-specific glycoproteomics analysis
    Article Snippet: Glycoproteins Recombinant Human PD-L1/B7-H1 Fc Chimera Protein overexpressed in Mouse myeloma (NS0) cell line (R&D Systems, Minneapolis, MN); Recombinant Human PD-L1/B7-H1 His-tag Protein overexpressed in Human embryonic kidney cell (HEK 293) cell line (R&D Systems), Human Haptoglobin, mixed type isolated from plasma (Athens Research & Technology, Athens, GA), Human Hemopexin isolated from plasma (Athens Research & Technology). .. His-tagged ProBDNF was expressed in suspension HEK293F cells (Invitrogen, Carlsbad, CA) and purified using Ni-NTA resin (Thermo, Rockford, IL) chromatography on AKTA Start FPLC system (GE Healthcare, Uppsala, Sweden) to > 90% purity as determined by Coomassie Blue stained SDS-PAGE as described 24 Lyophilized proteins were dissolved in 100 mM PBS at a concentration of 0.5 μg/ml and stored at −80°C. ..

    Fast Protein Liquid Chromatography:

    Article Title: Low collision energy fragmentation in structure-specific glycoproteomics analysis
    Article Snippet: Glycoproteins Recombinant Human PD-L1/B7-H1 Fc Chimera Protein overexpressed in Mouse myeloma (NS0) cell line (R&D Systems, Minneapolis, MN); Recombinant Human PD-L1/B7-H1 His-tag Protein overexpressed in Human embryonic kidney cell (HEK 293) cell line (R&D Systems), Human Haptoglobin, mixed type isolated from plasma (Athens Research & Technology, Athens, GA), Human Hemopexin isolated from plasma (Athens Research & Technology). .. His-tagged ProBDNF was expressed in suspension HEK293F cells (Invitrogen, Carlsbad, CA) and purified using Ni-NTA resin (Thermo, Rockford, IL) chromatography on AKTA Start FPLC system (GE Healthcare, Uppsala, Sweden) to > 90% purity as determined by Coomassie Blue stained SDS-PAGE as described 24 Lyophilized proteins were dissolved in 100 mM PBS at a concentration of 0.5 μg/ml and stored at −80°C. ..

    Article Title: Structure, dynamics, and molecular inhibition of the Staphylococcus aureus m 1 A22-tRNA methyltransferase TrmK.
    Article Snippet: .. Cells were allowed to grow for an additional 20 h, harvested by centrifugation at 6774g for 15 min, and stored at −20 C. Purification of SaTrmK All purification procedures were carried out at 4 C, and chromatographic steps employed an AKTA Start FPLC system (GE Lifesciences). .. Cells were allowed to thaw on ice for 20 min before being resuspended in buffer A (50 mM Hepes, 10 mM imidazole, 300 mM NaCl, pH 8.0) containing 0.2 mg mL−1 lysozyme, 0.05 mg mL−1 DNAse I, and half a tablet of EDTA-free cOmplete Protease Inhibitor Cocktail, disrupted in a high-pressure cell disruptor (Constant Systems), and centrifuged at 48,000g for 30 min to remove cell debris.

    Article Title: A 6-O-endosulfatase activity assay based on synthetic heparan sulfate oligomers.
    Article Snippet: A 6-O-endosulfatase activity assay based on synthetic heparan sulfate oligomers Julius Benicky1,2, Miloslav Sanda3,4, Aswini Panigrahi1,2, Jian Liu5, Zhangjie Wang5, Vijayakanth Pagadala6, Guowei Su6, Radoslav Goldman1,2,3,* 1Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC 20057, United States, 2Clinical and Translational Glycoscience Research Center, Georgetown University, Washington, DC 20057, United States, 3Department of Biochemistry and Molecular & Cell Biology, Georgetown University, Washington, DC 20057, United States, 4Max-Planck-Institut fuer Herzund Lungenforschung, Ludwigstrasse 43, Bad Nauheim 61231, Germany, 5Division of Chemical Biology and Medicinal Chemistry, Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, NC 27599, United States, 6Glycan Therapeutics, LLC, 617 Hutton Street, Raleigh, NC 27606, United States *Corresponding author: Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, 3970 Reservoir Rd NW, NRB-E207, Washington, DC 20057, United States.. Email: rg26@georgetown.edu

    Article Title: Functional and ligand binding studies of NAD(P)H hydrate dehydratase enzyme from vancomycin-resistant Staphylococcus aureus by NMR spectroscopic approach, including saturation transfer difference (STD-NMR) spectroscopy.
    Article Snippet: NADH and NADPH are labile coenzymes that undergo hydration by enzymatic reaction or by heat at 5,6 double bond, and convert into non-functional hydrates, NADHX and NADPHX, respectively.. The NAD(P)H hydrate dehydratase enzyme catalyzes the dehydration of S-NADHX/S-NADPHX at the expense of ATP, and thus contributes in the nicotinamide nucleotide repair process.. This enzyme is also known as “metabolite-proofreading enzyme”.

    Article Title: HLA class I signal peptide polymorphism determines the level of CD94/NKG2–HLA-E-mediated regulation of effector cell responses
    Article Snippet: In brief, constructs encoding the chimeric proteins with a His6 tag, utilizing the pHLSec-Avitag3 construct backbone, were transfected into Expi293F suspension cells according to the manufacturer’s protocols (Expi293 Expression System, Thermo Fisher Scientific). .. After 5 days of expression, the supernatant was harvested, clarified by centrifugation and filtration, and the tagged proteins purified by immobilized metal affinity chromatography utilizing a Ni-NTA column attached to an Akta start FPLC system (Cytiva Life Sciences) followed by size-exclusion chromatography using a Superdex S200 increase column, equilibrated with 150 mM NaCl 20 mM Tris pH 8. ..

    Article Title: BRCA1 interaction with Non-Canonical DNA Structures: Insights into Genome Maintenance and Disease Mechanisms
    Article Snippet: .. The Ni-NTA elution was immediately loaded onto a 5 mL HiTrap Heparin HP column (GE Healthcare, Chicago, IL, USA) that was equilibrated with 20 mM HEPES (pH 7.5), 30 mM NaCl, 5 mM TCEP, and 5% glycerol using an AKTA Start FPLC system (GE Healthcare). ..

    Article Title: Insight into novel biochemical features and function of novel antifungal GH16-β-glucanase from Bacillus velezensis.
    Article Snippet: β-Glucanases play a critical role in degrading fungal cell wall β-glucans, compromising fungal integrity and development.. Here we characterize BvlzGluc, a novel β-glucanase from Bacillus velezensis CECT 8237 with potent and specific activity against β-1,3-glucans.. The enzyme hydrolyzes yeast-derived β-glucan but is inactive against β-1,6and β-1,3/1,4-linked polysaccharides, such as laminarin and lichenin, indicating strict substrate specificity.

    Article Title: HLA class I signal peptide polymorphism determines the level of CD94/NKG2-HLA-E-mediated regulation of effector cell responses.
    Article Snippet: Human leukocyte antigen (HLA)-E binds epitopes derived from HLA-A, HLA-B, HLA-C and HLA-G signal peptides (SPs) and serves as a ligand for CD94/NKG2A and CD94/NKG2C receptors expressed on natural killer and T cell subsets.. We show that among 16 common classical HLA class I SP variants, only 6 can be efficiently processed to generate epitopes that enable CD94/NKG2 engagement, which we term ‘functional SPs’.. The single functional HLA-B SP, known as HLA-B/−21M, induced high HLA-E expression, but conferred the lowest receptor recognition.

    Staining:

    Article Title: Low collision energy fragmentation in structure-specific glycoproteomics analysis
    Article Snippet: Glycoproteins Recombinant Human PD-L1/B7-H1 Fc Chimera Protein overexpressed in Mouse myeloma (NS0) cell line (R&D Systems, Minneapolis, MN); Recombinant Human PD-L1/B7-H1 His-tag Protein overexpressed in Human embryonic kidney cell (HEK 293) cell line (R&D Systems), Human Haptoglobin, mixed type isolated from plasma (Athens Research & Technology, Athens, GA), Human Hemopexin isolated from plasma (Athens Research & Technology). .. His-tagged ProBDNF was expressed in suspension HEK293F cells (Invitrogen, Carlsbad, CA) and purified using Ni-NTA resin (Thermo, Rockford, IL) chromatography on AKTA Start FPLC system (GE Healthcare, Uppsala, Sweden) to > 90% purity as determined by Coomassie Blue stained SDS-PAGE as described 24 Lyophilized proteins were dissolved in 100 mM PBS at a concentration of 0.5 μg/ml and stored at −80°C. ..

    SDS Page:

    Article Title: Low collision energy fragmentation in structure-specific glycoproteomics analysis
    Article Snippet: Glycoproteins Recombinant Human PD-L1/B7-H1 Fc Chimera Protein overexpressed in Mouse myeloma (NS0) cell line (R&D Systems, Minneapolis, MN); Recombinant Human PD-L1/B7-H1 His-tag Protein overexpressed in Human embryonic kidney cell (HEK 293) cell line (R&D Systems), Human Haptoglobin, mixed type isolated from plasma (Athens Research & Technology, Athens, GA), Human Hemopexin isolated from plasma (Athens Research & Technology). .. His-tagged ProBDNF was expressed in suspension HEK293F cells (Invitrogen, Carlsbad, CA) and purified using Ni-NTA resin (Thermo, Rockford, IL) chromatography on AKTA Start FPLC system (GE Healthcare, Uppsala, Sweden) to > 90% purity as determined by Coomassie Blue stained SDS-PAGE as described 24 Lyophilized proteins were dissolved in 100 mM PBS at a concentration of 0.5 μg/ml and stored at −80°C. ..

    Concentration Assay:

    Article Title: Low collision energy fragmentation in structure-specific glycoproteomics analysis
    Article Snippet: Glycoproteins Recombinant Human PD-L1/B7-H1 Fc Chimera Protein overexpressed in Mouse myeloma (NS0) cell line (R&D Systems, Minneapolis, MN); Recombinant Human PD-L1/B7-H1 His-tag Protein overexpressed in Human embryonic kidney cell (HEK 293) cell line (R&D Systems), Human Haptoglobin, mixed type isolated from plasma (Athens Research & Technology, Athens, GA), Human Hemopexin isolated from plasma (Athens Research & Technology). .. His-tagged ProBDNF was expressed in suspension HEK293F cells (Invitrogen, Carlsbad, CA) and purified using Ni-NTA resin (Thermo, Rockford, IL) chromatography on AKTA Start FPLC system (GE Healthcare, Uppsala, Sweden) to > 90% purity as determined by Coomassie Blue stained SDS-PAGE as described 24 Lyophilized proteins were dissolved in 100 mM PBS at a concentration of 0.5 μg/ml and stored at −80°C. ..

    Centrifugation:

    Article Title: Structure, dynamics, and molecular inhibition of the Staphylococcus aureus m 1 A22-tRNA methyltransferase TrmK.
    Article Snippet: .. Cells were allowed to grow for an additional 20 h, harvested by centrifugation at 6774g for 15 min, and stored at −20 C. Purification of SaTrmK All purification procedures were carried out at 4 C, and chromatographic steps employed an AKTA Start FPLC system (GE Lifesciences). .. Cells were allowed to thaw on ice for 20 min before being resuspended in buffer A (50 mM Hepes, 10 mM imidazole, 300 mM NaCl, pH 8.0) containing 0.2 mg mL−1 lysozyme, 0.05 mg mL−1 DNAse I, and half a tablet of EDTA-free cOmplete Protease Inhibitor Cocktail, disrupted in a high-pressure cell disruptor (Constant Systems), and centrifuged at 48,000g for 30 min to remove cell debris.

    Article Title: HLA class I signal peptide polymorphism determines the level of CD94/NKG2–HLA-E-mediated regulation of effector cell responses
    Article Snippet: In brief, constructs encoding the chimeric proteins with a His6 tag, utilizing the pHLSec-Avitag3 construct backbone, were transfected into Expi293F suspension cells according to the manufacturer’s protocols (Expi293 Expression System, Thermo Fisher Scientific). .. After 5 days of expression, the supernatant was harvested, clarified by centrifugation and filtration, and the tagged proteins purified by immobilized metal affinity chromatography utilizing a Ni-NTA column attached to an Akta start FPLC system (Cytiva Life Sciences) followed by size-exclusion chromatography using a Superdex S200 increase column, equilibrated with 150 mM NaCl 20 mM Tris pH 8. ..

    Article Title: HLA class I signal peptide polymorphism determines the level of CD94/NKG2-HLA-E-mediated regulation of effector cell responses.
    Article Snippet: Human leukocyte antigen (HLA)-E binds epitopes derived from HLA-A, HLA-B, HLA-C and HLA-G signal peptides (SPs) and serves as a ligand for CD94/NKG2A and CD94/NKG2C receptors expressed on natural killer and T cell subsets.. We show that among 16 common classical HLA class I SP variants, only 6 can be efficiently processed to generate epitopes that enable CD94/NKG2 engagement, which we term ‘functional SPs’.. The single functional HLA-B SP, known as HLA-B/−21M, induced high HLA-E expression, but conferred the lowest receptor recognition.

    Binding Assay:

    Article Title: A 6-O-endosulfatase activity assay based on synthetic heparan sulfate oligomers.
    Article Snippet: A 6-O-endosulfatase activity assay based on synthetic heparan sulfate oligomers Julius Benicky1,2, Miloslav Sanda3,4, Aswini Panigrahi1,2, Jian Liu5, Zhangjie Wang5, Vijayakanth Pagadala6, Guowei Su6, Radoslav Goldman1,2,3,* 1Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC 20057, United States, 2Clinical and Translational Glycoscience Research Center, Georgetown University, Washington, DC 20057, United States, 3Department of Biochemistry and Molecular & Cell Biology, Georgetown University, Washington, DC 20057, United States, 4Max-Planck-Institut fuer Herzund Lungenforschung, Ludwigstrasse 43, Bad Nauheim 61231, Germany, 5Division of Chemical Biology and Medicinal Chemistry, Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, NC 27599, United States, 6Glycan Therapeutics, LLC, 617 Hutton Street, Raleigh, NC 27606, United States *Corresponding author: Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, 3970 Reservoir Rd NW, NRB-E207, Washington, DC 20057, United States.. Email: rg26@georgetown.edu

    Saline:

    Article Title: A 6-O-endosulfatase activity assay based on synthetic heparan sulfate oligomers.
    Article Snippet: A 6-O-endosulfatase activity assay based on synthetic heparan sulfate oligomers Julius Benicky1,2, Miloslav Sanda3,4, Aswini Panigrahi1,2, Jian Liu5, Zhangjie Wang5, Vijayakanth Pagadala6, Guowei Su6, Radoslav Goldman1,2,3,* 1Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC 20057, United States, 2Clinical and Translational Glycoscience Research Center, Georgetown University, Washington, DC 20057, United States, 3Department of Biochemistry and Molecular & Cell Biology, Georgetown University, Washington, DC 20057, United States, 4Max-Planck-Institut fuer Herzund Lungenforschung, Ludwigstrasse 43, Bad Nauheim 61231, Germany, 5Division of Chemical Biology and Medicinal Chemistry, Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, NC 27599, United States, 6Glycan Therapeutics, LLC, 617 Hutton Street, Raleigh, NC 27606, United States *Corresponding author: Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, 3970 Reservoir Rd NW, NRB-E207, Washington, DC 20057, United States.. Email: rg26@georgetown.edu

    Affinity Chromatography:

    Article Title: A 6-O-endosulfatase activity assay based on synthetic heparan sulfate oligomers.
    Article Snippet: A 6-O-endosulfatase activity assay based on synthetic heparan sulfate oligomers Julius Benicky1,2, Miloslav Sanda3,4, Aswini Panigrahi1,2, Jian Liu5, Zhangjie Wang5, Vijayakanth Pagadala6, Guowei Su6, Radoslav Goldman1,2,3,* 1Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC 20057, United States, 2Clinical and Translational Glycoscience Research Center, Georgetown University, Washington, DC 20057, United States, 3Department of Biochemistry and Molecular & Cell Biology, Georgetown University, Washington, DC 20057, United States, 4Max-Planck-Institut fuer Herzund Lungenforschung, Ludwigstrasse 43, Bad Nauheim 61231, Germany, 5Division of Chemical Biology and Medicinal Chemistry, Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, NC 27599, United States, 6Glycan Therapeutics, LLC, 617 Hutton Street, Raleigh, NC 27606, United States *Corresponding author: Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, 3970 Reservoir Rd NW, NRB-E207, Washington, DC 20057, United States.. Email: rg26@georgetown.edu

    Article Title: Functional and ligand binding studies of NAD(P)H hydrate dehydratase enzyme from vancomycin-resistant Staphylococcus aureus by NMR spectroscopic approach, including saturation transfer difference (STD-NMR) spectroscopy.
    Article Snippet: NADH and NADPH are labile coenzymes that undergo hydration by enzymatic reaction or by heat at 5,6 double bond, and convert into non-functional hydrates, NADHX and NADPHX, respectively.. The NAD(P)H hydrate dehydratase enzyme catalyzes the dehydration of S-NADHX/S-NADPHX at the expense of ATP, and thus contributes in the nicotinamide nucleotide repair process.. This enzyme is also known as “metabolite-proofreading enzyme”.

    Article Title: HLA class I signal peptide polymorphism determines the level of CD94/NKG2–HLA-E-mediated regulation of effector cell responses
    Article Snippet: In brief, constructs encoding the chimeric proteins with a His6 tag, utilizing the pHLSec-Avitag3 construct backbone, were transfected into Expi293F suspension cells according to the manufacturer’s protocols (Expi293 Expression System, Thermo Fisher Scientific). .. After 5 days of expression, the supernatant was harvested, clarified by centrifugation and filtration, and the tagged proteins purified by immobilized metal affinity chromatography utilizing a Ni-NTA column attached to an Akta start FPLC system (Cytiva Life Sciences) followed by size-exclusion chromatography using a Superdex S200 increase column, equilibrated with 150 mM NaCl 20 mM Tris pH 8. ..

    Article Title: Insight into novel biochemical features and function of novel antifungal GH16-β-glucanase from Bacillus velezensis.
    Article Snippet: β-Glucanases play a critical role in degrading fungal cell wall β-glucans, compromising fungal integrity and development.. Here we characterize BvlzGluc, a novel β-glucanase from Bacillus velezensis CECT 8237 with potent and specific activity against β-1,3-glucans.. The enzyme hydrolyzes yeast-derived β-glucan but is inactive against β-1,6and β-1,3/1,4-linked polysaccharides, such as laminarin and lichenin, indicating strict substrate specificity.

    Article Title: HLA class I signal peptide polymorphism determines the level of CD94/NKG2-HLA-E-mediated regulation of effector cell responses.
    Article Snippet: Human leukocyte antigen (HLA)-E binds epitopes derived from HLA-A, HLA-B, HLA-C and HLA-G signal peptides (SPs) and serves as a ligand for CD94/NKG2A and CD94/NKG2C receptors expressed on natural killer and T cell subsets.. We show that among 16 common classical HLA class I SP variants, only 6 can be efficiently processed to generate epitopes that enable CD94/NKG2 engagement, which we term ‘functional SPs’.. The single functional HLA-B SP, known as HLA-B/−21M, induced high HLA-E expression, but conferred the lowest receptor recognition.

    Expressing:

    Article Title: HLA class I signal peptide polymorphism determines the level of CD94/NKG2–HLA-E-mediated regulation of effector cell responses
    Article Snippet: In brief, constructs encoding the chimeric proteins with a His6 tag, utilizing the pHLSec-Avitag3 construct backbone, were transfected into Expi293F suspension cells according to the manufacturer’s protocols (Expi293 Expression System, Thermo Fisher Scientific). .. After 5 days of expression, the supernatant was harvested, clarified by centrifugation and filtration, and the tagged proteins purified by immobilized metal affinity chromatography utilizing a Ni-NTA column attached to an Akta start FPLC system (Cytiva Life Sciences) followed by size-exclusion chromatography using a Superdex S200 increase column, equilibrated with 150 mM NaCl 20 mM Tris pH 8. ..

    Article Title: HLA class I signal peptide polymorphism determines the level of CD94/NKG2-HLA-E-mediated regulation of effector cell responses.
    Article Snippet: Human leukocyte antigen (HLA)-E binds epitopes derived from HLA-A, HLA-B, HLA-C and HLA-G signal peptides (SPs) and serves as a ligand for CD94/NKG2A and CD94/NKG2C receptors expressed on natural killer and T cell subsets.. We show that among 16 common classical HLA class I SP variants, only 6 can be efficiently processed to generate epitopes that enable CD94/NKG2 engagement, which we term ‘functional SPs’.. The single functional HLA-B SP, known as HLA-B/−21M, induced high HLA-E expression, but conferred the lowest receptor recognition.

    Filtration:

    Article Title: HLA class I signal peptide polymorphism determines the level of CD94/NKG2–HLA-E-mediated regulation of effector cell responses
    Article Snippet: In brief, constructs encoding the chimeric proteins with a His6 tag, utilizing the pHLSec-Avitag3 construct backbone, were transfected into Expi293F suspension cells according to the manufacturer’s protocols (Expi293 Expression System, Thermo Fisher Scientific). .. After 5 days of expression, the supernatant was harvested, clarified by centrifugation and filtration, and the tagged proteins purified by immobilized metal affinity chromatography utilizing a Ni-NTA column attached to an Akta start FPLC system (Cytiva Life Sciences) followed by size-exclusion chromatography using a Superdex S200 increase column, equilibrated with 150 mM NaCl 20 mM Tris pH 8. ..

    Article Title: HLA class I signal peptide polymorphism determines the level of CD94/NKG2-HLA-E-mediated regulation of effector cell responses.
    Article Snippet: Human leukocyte antigen (HLA)-E binds epitopes derived from HLA-A, HLA-B, HLA-C and HLA-G signal peptides (SPs) and serves as a ligand for CD94/NKG2A and CD94/NKG2C receptors expressed on natural killer and T cell subsets.. We show that among 16 common classical HLA class I SP variants, only 6 can be efficiently processed to generate epitopes that enable CD94/NKG2 engagement, which we term ‘functional SPs’.. The single functional HLA-B SP, known as HLA-B/−21M, induced high HLA-E expression, but conferred the lowest receptor recognition.

    Size-exclusion Chromatography:

    Article Title: HLA class I signal peptide polymorphism determines the level of CD94/NKG2–HLA-E-mediated regulation of effector cell responses
    Article Snippet: In brief, constructs encoding the chimeric proteins with a His6 tag, utilizing the pHLSec-Avitag3 construct backbone, were transfected into Expi293F suspension cells according to the manufacturer’s protocols (Expi293 Expression System, Thermo Fisher Scientific). .. After 5 days of expression, the supernatant was harvested, clarified by centrifugation and filtration, and the tagged proteins purified by immobilized metal affinity chromatography utilizing a Ni-NTA column attached to an Akta start FPLC system (Cytiva Life Sciences) followed by size-exclusion chromatography using a Superdex S200 increase column, equilibrated with 150 mM NaCl 20 mM Tris pH 8. ..

    Article Title: HLA class I signal peptide polymorphism determines the level of CD94/NKG2-HLA-E-mediated regulation of effector cell responses.
    Article Snippet: Human leukocyte antigen (HLA)-E binds epitopes derived from HLA-A, HLA-B, HLA-C and HLA-G signal peptides (SPs) and serves as a ligand for CD94/NKG2A and CD94/NKG2C receptors expressed on natural killer and T cell subsets.. We show that among 16 common classical HLA class I SP variants, only 6 can be efficiently processed to generate epitopes that enable CD94/NKG2 engagement, which we term ‘functional SPs’.. The single functional HLA-B SP, known as HLA-B/−21M, induced high HLA-E expression, but conferred the lowest receptor recognition.

    Protein Purification:

    Article Title: Insight into novel biochemical features and function of novel antifungal GH16-β-glucanase from Bacillus velezensis.
    Article Snippet: β-Glucanases play a critical role in degrading fungal cell wall β-glucans, compromising fungal integrity and development.. Here we characterize BvlzGluc, a novel β-glucanase from Bacillus velezensis CECT 8237 with potent and specific activity against β-1,3-glucans.. The enzyme hydrolyzes yeast-derived β-glucan but is inactive against β-1,6and β-1,3/1,4-linked polysaccharides, such as laminarin and lichenin, indicating strict substrate specificity.



    Similar Products

    akta start fplc system  (Cytiva Europe)
    96
    Cytiva Europe akta start fplc system
    Akta Start Fplc System, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/akta+start+fplc+system/AKTA+start+chromatography+system/pm41500280-79-18-22
    Average 96 stars, based on 1 article reviews
    akta start fplc system - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    akta fplc system  (Cytiva Europe)
    96
    Cytiva Europe akta fplc system
    Akta Fplc System, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/akta+start+fplc+system/AKTA+start+chromatography+system/pmc12630136-98-23-26
    Average 96 stars, based on 1 article reviews
    akta fplc system - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    akta fplc chromatography systems  (Cytiva Europe)
    96
    Cytiva Europe akta fplc chromatography systems
    Akta Fplc Chromatography Systems, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/akta+start+fplc+system/AKTA+start+chromatography+system/pmc12472053-131-4-8
    Average 96 stars, based on 1 article reviews
    akta fplc chromatography systems - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    akta purifier fplc system  (Cytiva Europe)
    96
    Cytiva Europe akta purifier fplc system
    Akta Purifier Fplc System, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/akta+start+fplc+system/AKTA+start+chromatography+system/pm40000726-171-55-59
    Average 96 stars, based on 1 article reviews
    akta purifier fplc system - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    akta fast protein liquid chromatography instrument  (Cytiva Europe)
    96
    Cytiva Europe akta fast protein liquid chromatography instrument
    Akta Fast Protein Liquid Chromatography Instrument, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/akta+start+fplc+system/AKTA+start+chromatography+system/pm39912667-68-25-31
    Average 96 stars, based on 1 article reviews
    akta fast protein liquid chromatography instrument - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier

    Image Search Results